Protein
tRNA(Ile)-lysidine synthase
Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Function
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
Similarity
Belongs to the tRNA(Ile)-lysidine synthase family.
Sequence
MSLFHLLRRSVTVAVMSESAPNHTSARRLTQPLEPFVGRQVLVGVSGGADSVGLLRALLAVGALPAVAHLDHALRPESVDDAAWVSDLCARLGVPCEVTRIDVGAVAARRNWNLEAARRLRYDVLSRAAKHSGAEVILTAHTRRDQAETVLMELLRGEGRIRGIPPQRGRVRRPWLNVGRAEIETYLRGLGQDWREDASNADPRFTRAWLRREVMPVLLTRFPAAETALAQVAELGAEDDAALQALAARLTPHTPLDRQPPAVLRRWLAAELRSGGLDFTAEQLRDLAGALNAGQTRHLTLPTGRDVTVTGGHLYTAPCDWPEPDFPLPPDWTRRTRQAGDRIRLAGGTRKLSDVLTDAHLPRAERDRVPLLTDEHGAVQWVGVQPPLWAVGARESLGLPADPLHAAMGEALALAQQAAERQEVPVGAVVLNADGEIVGRGRNTSREDGDMTCHAELAALREAAAGLGTPYLSDCTLVVTLEPCPMCLGAALEARIGHIVYGAANPKAGALGGVSDLLADHWGWRPTVQGGVRAGEAARLLREVFGEVRRRSADTPQTPNAETPAPRSSRSTSASGKPTMLE