Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.

Belongs to the tRNA(Ile)-lysidine synthase family.

456 amino acids

MGLSPSNISKLFEPFGFEQAKAVIAAVSGGSDSLGLLFLVRDYMAMLQNPPRLIAVTVDHQLRAESKAEAENVGLLCRQYGIEHRILVWDEAKPTSGLAAAARTARYRLLVQAARDVGGAFIVTGHTQDDQIETFLMRKERSAHAEARGLAAMSLRSLLDGLGLEGSVELDRPLLSVSRQTLRDELRGRGINWVDDPSNANTEYERPRIRHGIAAEADRQTVLDQIAEAGAARERDNAALIAALGNPASLRADANGALLVDPQLYAALPGNARRLFSGLLAAIAGGRRFLPGDSERSRIERVLSGDDDTHRLTVFGALIERGDSGSPHRFLREKRNLPKLHLEPGKPIVWDGRFRFLNEGMMDFELAAPGRQELADFLKSQNIEIESRKREALLVSPALYREGRLFALLFLQDGEFQQDIHIERHFAIFDHVLPGHDFDLARAVEARIGRICAEMS

50.4 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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