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ribU

Gene
ribU
Protein
Riboflavin transporter RibU
Organism
Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
Length
211 amino acids
Function
Substrate-binding (S) component of an energy-coupling factor (ECF) ABC-transporter complex. Mediates riboflavin uptake, may also transport FMN and roseoflavin. Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (Probable). Expression of the complex plus RibU in E.coli allows riboflavin uptake.
Similarity
Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
Mass
23.644 kDa
Sequence
MRSLFFGIGKSIGNFFQIVKIWRNFFMTNTRKLAYIAILSAVSFLLLYFSFPLIPAADFLKVDFSILPVLIALVIFDFKSAIGVLLLRSLLKLLLNNGGPGSMIGLPMNFVALGVFVWGLSYFWKKNQTSKNYILGSVLGTILLTVAMVVLNYIYAVPLYAKFANFDIAQFIGLYKYLFAMVVPFNLLEGLIFSVAFALIYAPLKSILVKL

Gene
ribU
Protein
Riboflavin transporter RibU
Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Length
206 amino acids
Function
Mediates riboflavin uptake, may also transport FMN and roseoflavin (By similarity). Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins. Uptake of riboflavin into proteosomes containing EcfA1A2T and RibU has been demonstrated. Uptake requires hydrolyzable Mg-ATP.
Similarity
Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
Mass
22.972 kDa
Sequence
MSKTRRMVLIAMLAALSTILLLPILQFPLLPGIDFMKVELSIIPVLIGVFTLGLGDGFIILFIRSVLWYLLFNQGPSTWIGVPMNFVALGIFMAIVWFFTKKKFSIKNYTVGIVLATIASVLVMMVLNVFYALPLYRLAAGFDVDKIFAGATHLFNMGSLSVTLNPTYLLTVVLPFNALQYIIFALVFGLIVTVFKKNKVVKFYNA

Gene
ribU
Protein
Riboflavin transporter RibU
Organism
Lactococcus lactis subsp. cremoris (strain NZ9000)
Length
206 amino acids
Function
Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (By similarity). Mediates riboflavin uptake, probably also transports FMN and roseoflavin.
Similarity
Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
Mass
22.972 kDa
Sequence
MSKTRRMVLIAMLAALSTILLLPILQFPLLPGIDFMKVELSIIPVLIGVFTLGLGDGFIILFIRSVLWYLLFNQGPSTWIGVPMNFVALGIFMAIVWFFTKKKFSIKNYTVGIVLATIASVLVMMVLNVFYALPLYRLAAGFDVDKIFAGATHLFNMGSLSVTLNPTYLLTVVLPFNALQYIIFALVFGLIVTVFKKNKVVKFYNA

Gene
ribU
Protein
Riboflavin transporter RibU
Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Length
196 amino acids
Function
Probable riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins and transport it into cells (By similarity).
Similarity
Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
Mass
22.268 kDa
Sequence
MSIIPVTRVQRTTLIAILSAISFGLMLFPQVPIIPSADFLKLDFSIVPVVIGLYWLNYSASLWVILIRTLLKLILANEGVNTYLGLPVNLLVVLAFITVLKITMPNLEQYSNWQKKILPLISSTFVMTIVAIVINWFVAIPLYARFANFDIAKFIGLKNYFIGMVLPFNLIEGIIWFVVSMIILRAIQPLQRRFHS

Gene
ribU
Protein
Riboflavin transporter RibU
Organism
Staphylococcus aureus (strain TCH60)
Length
189 amino acids
Function
Mediates riboflavin uptake, may also transport FMN and roseoflavin. Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (By similarity).
Similarity
Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
Mass
21.009 kDa
Sequence
MNGRRKLNMQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIIVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTKSLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLKRI

Gene
ribU
Protein
Riboflavin transporter RibU
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
183 amino acids
Function
Substrate-binding (S) component of an energy-coupling factor (ECF) ABC-transporter complex. Mediates riboflavin uptake, may also transport FMN and roseoflavin. Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (Probable). Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.
Similarity
Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
Mass
20.015 kDa
Sequence
MSSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLVKDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALGLNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKLETVEGRSNNG