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phaE

Gene
phaE
Protein
Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Organism
Thiocystis violacea
Length
364 amino acids
Function
Polymerizes D(-)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB) which consists of thousands of hydroxybutyrate molecules linked end to end. This subunit has no catalytic activity but enhances the activity of PhaC, the catalytic subunit.
Similarity
Belongs to the PHA/PHB synthase family. Type III PhaE subfamily.
Mass
41.387 kDa
Sequence
MSNDSFFNNDWLELQRKYWDSWSEMGRKAMGLENQQTLTTPWEGALDHWWKAMSPATPDFSKTFMEKMMEQGKNFFRMAETFANTPEDTTATNGLTWWTKALEDMQKQFSGSLDDGGNSMQRMMSFWELPIDNWQRMMSSMSPMPGDMLRNMPHEQLKDRSGRALSAPGLGYTREEQSQYHQLTRTAMDYQAALQEYTGFYSQLGMKSVERMGDFIQGVIDSGKSIDSARTLYDNWISCCETVYAAEVATPEYAQIHGRLVNAQMALKRRMAIMVDENLGAMNMPTRSELRTLQDRLQETRRDNKQLHRALHALEKQVAALSGKTPTTALKAPAPATKATEKPATRATTRRKTAAKPTGGTADD

Gene
phaE
Protein
Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Organism
Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D)
Length
357 amino acids
Function
Polymerizes D(-)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB) which consists of thousands of hydroxybutyrate molecules linked end to end (PubMed:7957260). This subunit has no catalytic activity but enhances the activity of PhaC, the catalytic subunit, 100-fold (PubMed:9888824).
Similarity
Belongs to the PHA/PHB synthase family. Type III PhaE subfamily.
Mass
40.524 kDa
Sequence
MSNTNFFNDDWLELQRKYWDNWTDMSRKAMGLDSASSSATTPWEAAIDQWWKAMAPAAPDLSRSFMEKMMEQGKNFFRLADTFAKRADEGNAGNGLELWTKTLEDMQKRFSGSLDDGGNTMQRLMSFWELPLDNWQRMMSSMSPMPGDMLRNMPHEQFKDSLDRALSAPGLGYTREEQSQYQELMRSAMEYQAALQEYTNVYTKLGMKSVEHMGSYIQGVIDSGKTIDSARALYDNWVACCEGAYADEVATPEYARIHGRLVNAQMALKKRMSILVDENLGALNMPTRSELRTLQDRLQETRRENKALRHSLHSLERRVAALAGEEPATKPATALRSPAPAAKAPARRRTTKTNPAD

Gene
phaE
Protein
Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Length
330 amino acids
Function
When expressed in E.coli with Synechocystis PhaC and C.necator PhaA and PhaB, confers the ability to synthesize up to 13% (w/w) poly(3-hydroxybutyrate) (PHB) depending on the carbon source; all 4 genes are necessary for PHB production (PubMed:9683655). Cell-free in vitro coexpression with PhaE gives a heterodimer able to polymerize 3-hydroxybutyrate-CoA (PubMed:25629766). This subunit has no catalytic activity but enhances the activity of PhaC, the catalytic subunit (By similarity).
Similarity
Belongs to the PHA/PHB synthase family. Type III PhaE subfamily.
Mass
38.047 kDa
Sequence
MESTNKTWTELMTPLSQFWLESSSQAWKNWFDLMAKGGAGAMMGSAPQSFESLPQQFLQSQQFYGELLKLSFEAWQSLWPKLDNGSAPGAVQGYLKQLQTQIEQYTATTQALQGDMDGLWQCYIKEVQRFSQLWLSTWQSSVAPLGKLPTGDIHAWLDLNNLYGDALYNKNLSSFMRSPLLGPSREMNGKLLRAFDDWVKLSQAMADYQLLEADIQYRGFAALMEDLLARAKEDKPVKTWKEFQQRWAIAADQVFEEAFCEEKNLKVRGKFINALNRYRIQQQEILEAWLKMLNLPTRSEVDEIHQTIYQLRKEVKSLKKRLGETEANPG

Gene
phaE
Protein
Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Organism
Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)
Length
182 amino acids
Function
Involved in the production of polyhydroxyalkonic acids (PHAs), which are water-insoluble biopolymers used as intracellular energy reserve material when cells grow under conditions of nutrient limitation. PHAs are composed primarily of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV). Required for the production of poly-beta-hydroxybutyrate (PHB) and poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) (PHBV).
Similarity
Belongs to the PHA/PHB synthase family.
Mass
20.452 kDa
Sequence
MSQQKGDEWTMYAAEMNETMLAALERNVEAQTQFVESWLDALEETPEMSTETISEGLNGYARAYEVWMNAAEQQFERASDAFEGEDVSANEFRDIWLNSANEAFKEVMGTSAFAAATGQTVEDALEMQREVDEAAQSTLRTLGFATEGDIDEVAERLVELERRQHAVETKLDRLLDAMDVEG

Gene
phaE
Protein
Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Organism
Haloarcula hispanica (strain ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755)
Length
181 amino acids
Function
Involved in the production of polyhydroxyalkonic acids (PHAs), which are water-insoluble biopolymers used as intracellular energy reserve material when cells grow under conditions of nutrient limitation. PHAs are composed primarily of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV). Required for the production of poly-beta-hydroxybutyrate (PHB) and poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) (PHBV).
Similarity
Belongs to the PHA/PHB synthase family.
Mass
20.61 kDa
Sequence
MSNTNNIQEEWTEMVEEMNNAVADSMEQNMKAQAAFVESWADAVEDTIPKEEDLADGMDGYNRAYEEWIDAAEQMVERSTDAAQGEDVDPAEFRDIWLQSANEAFKHVMGTSAFAAANGQLVESMMEMQQEADDLSQDALEQMGFPTRNDVDEVAERLIELERRQHAVEQKLDRVLEHLEE

Gene
phaE
Protein
Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Length
181 amino acids
Function
Involved in the production of polyhydroxyalkonic acids (PHAs), which are water-insoluble biopolymers used as intracellular energy reserve material when cells grow under conditions of nutrient limitation. PHAs are composed primarily of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV). Required for the production of poly-beta-hydroxybutyrate (PHB) and poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) (PHBV).
Similarity
Belongs to the PHA/PHB synthase family.
Mass
20.64 kDa
Sequence
MSNTNNIQEEWTEMVEEMNNAVADSMEQNMKAQAAFVESWADAVEDTIPEQDDLADGMDGYNRAYEEWMDAAEQMVERSTDAAQGEDVDPAEFRDIWLQSANEAFKHVMGTSAFAAANGQLVESMMEMQQEADDLSQDTLEQLGFPTRNDVDEIAERLIELERRQHAVEQKLDRVLEHLEE

Gene
phaE
Protein
Probable K(+)/H(+) antiporter subunit E
Organism
Rhizobium meliloti (strain 1021)
Length
161 amino acids
Function
Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development.
Similarity
Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.
Mass
18.24 kDa
Sequence
MRTWFPYPLLSIALLLMWLLLSQSVTPGSIVLGLVVSTVLAWVTLNLQPARSRLHRWSRIAGFILRVVGDVIRSNIAVTLIILRAGRRPVNAGFMTVSLDLDDENALALLACVVTATPGTAWLEYDRRQKILLFHVLDIENEDLWRKTITRYAADLKEIFE