Protein expression services for phaE | Poly(3-hydroxyalkanoate) polymerase subunit PhaE
Description
When expressed in E.coli with Synechocystis PhaC and C.necator PhaA and PhaB, confers the ability to synthesize up to 13% (w/w) poly(3-hydroxybutyrate) (PHB) depending on the carbon source; all 4 genes are necessary for PHB production (PubMed:9683655). Cell-free in vitro coexpression with PhaE gives a heterodimer able to polymerize 3-hydroxybutyrate-CoA (PubMed:25629766). This subunit has no catalytic activity but enhances the activity of PhaC, the catalytic subunit (By similarity).
Family
Belongs to the PHA/PHB synthase family. Type III PhaE subfamily.
Species
Synechocystis sp. (strain PCC 6803 / Kazusa)
Length
330 amino acids
Sequence
MESTNKTWTELMTPLSQFWLESSSQAWKNWFDLMAKGGAGAMMGSAPQSFESLPQQFLQSQQFYGELLKLSFEAWQSLWPKLDNGSAPGAVQGYLKQLQTQIEQYTATTQALQGDMDGLWQCYIKEVQRFSQLWLSTWQSSVAPLGKLPTGDIHAWLDLNNLYGDALYNKNLSSFMRSPLLGPSREMNGKLLRAFDDWVKLSQAMADYQLLEADIQYRGFAALMEDLLARAKEDKPVKTWKEFQQRWAIAADQVFEEAFCEEKNLKVRGKFINALNRYRIQQQEILEAWLKMLNLPTRSEVDEIHQTIYQLRKEVKSLKKRLGETEANPG
Mass
38 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make phaE using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)
Order Here