Protein
Modification methylase SmaI
Organism
Serratia marcescens
Function
This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.
Sequence
MKKHSNNLDLFDQTEECLESNLRSCKIIVGDAREAVQGLDSEIFDCVVTSPPYWGLRDYGNGGQIGAEDNINDYIKDLVDLFRDVRRTLKDDGTLWLNIGDSYTSGGRTWRDKDDKNKGRAMSYRPPTPEGLKPKDLIGVPWRLAFALQNDGWYLRTDIIWNKPNCQPESVRDRPTRSHEYIFLLSKGKKYYYDWESIKEPASDPKMDKKNRRTVWNINTEPYPGSHFAVFPRAMARLCVLAGSRPGGKVLDPFFGSGTTGVVCQELDRECVGIELNEEYASLAKERILRRR