This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease.

Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

292 amino acids

MKKHSNNLDLFDQTEECLESNLRSCKIIVGDAREAVQGLDSEIFDCVVTSPPYWGLRDYGNGGQIGAEDNINDYIKDLVDLFRDVRRTLKDDGTLWLNIGDSYTSGGRTWRDKDDKNKGRAMSYRPPTPEGLKPKDLIGVPWRLAFALQNDGWYLRTDIIWNKPNCQPESVRDRPTRSHEYIFLLSKGKKYYYDWESIKEPASDPKMDKKNRRTVWNINTEPYPGSHFAVFPRAMARLCVLAGSRPGGKVLDPFFGSGTTGVVCQELDRECVGIELNEEYASLAKERILRRR

33.5 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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