SEN1

Helicase SEN1

2231 amino acids

ATP-dependent 5'->3' DNA/RNA helicase required for the expression and maturation of diverse classes of non-protein-coding RNAs like precursor tRNAs, rRNAs and small nuclear (snRNA) and nucleolar (snoRNA) RNAs. Directs RNA polymerase II transcription termination on snoRNAs as well as on several short protein-coding genes. May also play a role in transcription-coupled nucleotide excision repair.

Belongs to the DNA2/NAM7 helicase family.

252.497 kDa

MNSNNPDNNNSNNINNNNKDKDIAPNSDVQLATVYTKAKSYIPQIEQVYQGTNPNIQEAKLLGELLQVLAEVPKGTHLFCDPILEPISIFSLTIFSFNEEATATWLKNHFNPILSVCDKCILNFARGKCKMLQHFAIQRHVPHEHVAKFNDIVCQWRVEAVFPILRNISVNDNTGINITNEIETAMYECLCNPHMLRLNKQLKATFEAIFKFFYDTKHRLLDVTNPLSIKTFISGVIFCWCEGSKEENEWSRAFLKDLYSRNFHINLSNLTPDIIEEVYIHILFLQNPANWTEIVVSQFWSRLLPVFNLFDKDVFIEYFQVPKNVESLKKTFKFPLEPIFKMWYNHLSKSYHDKPLDFLLRGLTMFLNKFGSEFWSKIEPFTFHSILDIIFNRDSFPIKLIKIQDNPIVEHQTEVYFQLTGSVTDLLSWTLPFYHALSPSKRIQMVRKVSMAFLRIIANYPSLKSIPKACLMNSATALLRAVLTIKENERAMLYKNDEFETVLLTKTDSRALLNNPLIQDIIIRSASNPNDFYPGLGAASASVATSTMMVLAECIDFDILLLCHRTFKLYSGKPISEIPISTNVLENVTNKIDLRSFHDGPLLAKQLLVSLKNINGLLIVPSNTAVAEAHNALNQKFLLLSTRLMEKFADILPGQLSKILADEDASQGFWSCIFSSDKHLYQAATNILYNTFDVEGRLEGILAILNSNLTVNLKNINVMLQRLINCEFYEPCPRAVRVLMDVVSAFVDPISGVFANFQTLKSQNTEKEFLKFWESCWLFLDTIYKFTLKWASKYDYSELENFTKDTLDLSRSLVDSFREFSDILHDQTKNLLLNVLETFKNMLYWLRLSDEVLLESCVRLIISTSDLAHEKHVKVDDSLVEMMAKYASKAKRFSNKLTEQQASEILQKAKIFNKALTEEVATEAENYRKEKELSRLGKVIDLTDSVPASPSLSPSLSSTIASSSAESRADYLQRKALSSSITGRPRVAQPKITSFGTFQSSANAKLHRTKPVKPLSKMELARMQLLNNRVVHPPSAPAFHTKSRGLSNKNDDSSSEESDNDIESARELFAIAKAKGKGIQTVDINGKVVKRQTAAELAKQELEHMRKRLNVDMNPLYEIILQWDYTRNSEYPDDEPIGNYSDVKDFFNSPADYQKVMKPLLLLESWQGLCSSRDREDYKPFSIIVGNRTAVSDFYDVYASVAKQVIQDCGISESDLIVMAYLPDFRPDKRLSSDDFKKAQHTCLAKVRTLKNTKGGNVDVTLRIHRNHSFSKFLTLRSEIYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVNVDAAEIETVKKSYKLNTSQAEAIVNSVSKEGFSLIQGPPGTGKTKTILGIIGYFLSTKNASSSNVIKVPLEKNSSNTEQLLKKQKILICAPSNAAVDEICLRLKSGVYDKQGHQFKPQLVRVGRSDVVNVAIKDLTLEELVDKRIGERNYEIRTDPELERKFNNAVTKRRELRGKLDSESGNPESPMSTEDISKLQLKIRELSKIINELGRDRDEMREKNSVNYRNRDLDRRNAQAHILAVSDIICSTLSGSAHDVLATMGIKFDTVIIDEACQCTELSSIIPLRYGGKRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRMEKNSSPYLLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFDIISGRQEQNAKTMSYTNMEEIRVAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKLWRDLIEDAKDRSCLAYACSGFLDPRNNRAQSILRKFNVPVPSEQEDDYKLPMEYITQGPDEVKSNKDTKKRRVVDEGEEADKAVKKKKKEKKKEKKKSKADDKKKNNKKAESPSTSSGTKKKSSIFGGMSVPSAVVPKTFPDVDSNKKAAAVVGKKKNNKHVCFSDDVSFIPRNDEPEIKVTRSLSSVLKEKQLGLKETRTISPPEISNNEDDDDEDDYTPSISDSSLMKSEANGRNNRVASHNQNFSASIYDDPQVSQAKQTQVPAAITKHRSSNSVLSGGSSRILTASDYGEPNQNGQNGANRTLSQHVGNANQYSTAPVGTGELHETLPAHPQDSYPAEAEDPYDLNPHPQPQSSAFKGPGSGPTGTRNSSRRNASSSPFIPKKRKPRS

sen1

Helicase sen1

1687 amino acids

ATP-dependent 5'->3' DNA/RNA helicase required for the expression and maturation of diverse classes of non-protein-coding RNAs like precursor tRNAs, rRNAs and small nuclear (snRNA) and nucleolar (snoRNA) RNAs. Directs RNA polymerase II transcription termination on snoRNAs as well as on several short protein-coding genes. May also play a role in transcription-coupled nucleotide excision repair (By similarity).

Belongs to the DNA2/NAM7 helicase family.

192.548 kDa

MAENLSDQDCFEKLSSSKEGQHWFCSGLLTQYIQPTFFWFAHDETPSFKWVLNAYHERLRSCTSCIQAYYELRNESLAKGSYSFTGFSITDLQEKWNKWDIIRVLEDFKALEDETIDFTSLPCLIFETLLNPKLFTCKNIYKNAIDAFNGLLSDWCSNIFLPGYLLFFYEASHPDVLEWVHSFFKENTEIRISSATVDAVFNTVVFESQNSSDSDCNFVSISSPEFWERTYMFLELLPLQSITAACESILKDYLLNLVKTSESLSSQQMSCLRICCNSSSFWSAADSFKEVSSFLKNLLKNVTATPFEVSDMNWAYIIASFFRTCLNDFVSVFPEWLKGFVEEKRTIGFIIYSVLDALFDLLSLDYSSPSFLNNTLSLMNANSITILQDYPEYMLKLRLHSLLYDIAFISWSHKAITKNPSFVFDPSYELSPFWKLDNLQEEKISDVLFSKISSCCFAHDIEISENSTPSGTMVQFAELWQVMSEYISGFLKGFSEKSSTEISNMLGDSSKFDTVVSFLLSPTQPLYVSAFHIVQIITNCTKNRNEALKKLVAMDFRGIVHGLADAVLNWQSILSFFPALRIMRFLSITNKSLSSDNSAFTENDIPTLGAYWQCIWNILDLVFSNVARWSLNNPADTVKALMKLTLKFVDDLFQNDGIFIKLLAKFDSLILLGETSESLFSFIMWLKINDLELRGIVINSLCKLFTKFSNFDYLFEDRTVTFLTDFIIRKQKAHLSADQCKQLANVLTQASPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPTPQITVKQNTTKSSSAPRMGMLEQLKQEYLTKRNFESKLKSSAVSSRKPTFNEVKPANLLAEDLSDNEDDIDRKQGLFSLAKANKIPEIRQQERRQVQLLSNSTIKMHPSQIRMMTNRNVANVKARLFPSMTDFYKEILSWEPANQSPNPVLKFHKLDGKIIDSFKTVEHYMEVLQPMIFMECWSQIQSTKLDLKFSPVEGIMVERTAVNNFVDIGVSVAPKDLYGYPLYDTEVVSLAFNKEDASSMKGLCCFAKVERIVRQTNGVLVVLRTLPSMEILNKLQGNCALWFLKLTNLATFTRQYAGIRGLPYFHLADDIIRARPCSQPVKHSSSEIKAAMKRYQVNEPQAKAIMCALDNNGFTLIQGPPGTGKTKTIIGIISALLVDLSRYHITRPNQQSKSTESKQQILLCAPSNAAVDEVLLRLKRGFLLENGEKYIPRVVRIGNPETINVSVRDLSLEYQTEKQLLEVNQGAIDLGSLQELTRWRDTFYDCIQKIEELEKQIDVARDVAEDTKSLGKELQNKINEKNLAEQKVEELQSQSFTKNKEVDLLRKKAQKAILKQADVVCATLSGSGHDLVAHSSLNFSTVIIDEAAQAVELDTIIPLRYGAKKCILVGDPNQLPPTVLSKKAASLNYSQSLFVRIQKNFSNQMCLLSIQYRMHPDISHFPSKKFYDSRLEDGDNMAEKTQQVWHVNPKFTQYRLFDVRGKERTSNTMSTYNLEEVEYLVNMVDELLNKFPDVNFTGRIGVITPYRSQLHELRRAFKVKYGKSFMSTIDIQTVDGFQGQEKDIIFFSCVKSYSKHGIGFLRDFRRLNVALTRARSSLLIIGNMETLKTDDLWGSLVDDALSRKLVESPHIDSEGRLITISRTSEKRMKNEEFVEPPSKKLANSEPSKEIRQRS

SEN1

tRNA-splicing endonuclease subunit Sen2-1

237 amino acids

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage (By similarity).

Belongs to the tRNA-intron endonuclease family.

27.359 kDa

MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCCFGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLENEVDLWRSMSSFKQDFAILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGGNDRLKVWSDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDETRT