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queF-L

Gene
queF-L
Protein
Archaeosine synthase
Organism
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Length
109 amino acids
Function
Is responsible for the final step in the biosynthesis of archaeosine, a modified nucleoside present in the dihydrouridine loop (D-loop) of archaeal tRNA (PubMed:28383498, PubMed:22032275). Catalyzes the conversion of 7-cyano-7-deazaguanine (preQ0)-modified tRNA to archaeosine-tRNA, transforming a nitrile group to a formamidine group. Can use neither glutamine nor asparagine as amino donor in vitro, is only able to utilize free ammonium (PubMed:28383498). However, the enzyme might function in vivo with a partner that serves to generate ammonium.
Similarity
Belongs to the archaeosine synthase type 2 family.
Mass
12.055 kDa
Sequence
MLKVSKSPSLVRLKTRGESVCPISKTVDSFEVSVEYIPRGAVLAIEEFKKMVDSYRGREILHEELAVDLLEKVKAAVNPPYVKVTVKSYYIGVEVEVVAESGGVPPVYI