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pikAV

Gene
pikAV
Protein
Thioesterase PikA5
Organism
Streptomyces venezuelae
Length
281 amino acids
Function
Involved in the biosynthesis of 12- and 14-membered ring macrolactone antibiotics such as methymycin, neomethymycin, narbomycin and pikromycin (PubMed:9770448, PubMed:10421766). Responsible for removing mis-formed acyl moieties (aberrant decarboxylation) that are bound to the PKS and could block it (PubMed:10421766, PubMed:12368286). Catalyzes the cleavage of methylmalonyl-[acp] (PubMed:12368286). It exhibits some acyl-group specificity, and catalyzes the cleavage of propionyl and butyryl derivatives faster than acetyl malonyl or methylmalonyl derivatives (PubMed:12368286).
Similarity
Belongs to the thioesterase family.
Mass
31.7 kDa
Sequence
MTDRPLNVDSGLWIRRFHPAPNSAVRLVCLPHAGGSASYFFRFSEELHPSVEALSVQYPGRQDRRAEPCLESVEELAEHVVAATEPWWQEGRLAFFGHSLGASVAFETARILEQRHGVRPEGLYVSGRRAPSLAPDRLVHQLDDRAFLAEIRRLSGTDERFLQDDELLRLVLPALRSDYKAAETYLHRPSAKLTCPVMALAGDRDPKAPLNEVAEWRRHTSGPFCLRAYSGGHFYLNDQWHEICNDISDHLLVTRGAPDARVVQPPTSLIEGAAKRWQNPR