Protein
N-acetylmuramic acid 6-phosphate phosphatase
Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Function
Specifically catalyzes the dephosphorylation of N-acetylmuramate 6-phosphate (MurNAc-6P) to MurNac. Is involved in peptidoglycan recycling as part of a cell wall recycling pathway that bypasses de novo biosynthesis of the peptidoglycan precursor UDP-MurNAc. Plays a role in intrinsic resistance to fosfomycin, which targets the de novo synthesis of UDP-MurNAc. Shows a very low activity on GlcNAc-6P, and neither alpha-1-phosphorylated MurNAc, GlcNAc, or glucose nor glucosamine-6P or glucose-6P can be used as a substrate.
Similarity
Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family. Phosphatase MupP subfamily.
Sequence
MRLRAVLFDMDGTLLDTAPDFIAICQAMLAERGLPAVDDNLIRGVISGGARAMVATAFAMDPEADGFEALRLEFLERYQRDCAVHSKLFEGMAELLADIEKGNLLWGVVTNKPVRFAEPIMQQLGLAERSALLICPDHVKNSKPDPEPLILACKTLNLDPASVLFVGDDLRDIESGRDAGTRTAAVRYGYIHPEDNPNNWGADVVVDHPLELRKVIDSALCGC