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mokH

Gene
mokH
Protein
Transcription factor mokH
Organism
Monascus pilosus
Length
455 amino acids
Function
Transcription factor that regulates the gene cluster that mediates the biosynthesis of monakolin K, also known as lovastatin, and which acts as a potent competitive inhibitor of HMG-CoA reductase (PubMed:18578535, PubMed:19693441, PubMed:19968298). Monakolin K biosynthesis is performed in two stages (PubMed:19693441). The first stage is catalyzed by the nonaketide synthase mokA, which belongs to type I polyketide synthases and catalyzes the iterative nine-step formation of the polyketide (PubMed:18578535, PubMed:19693441). This PKS stage is completed by the action of dehydrogenase mokE, which catalyzes the NADPH-dependent reduction of the unsaturated tetra-, penta- and heptaketide intermediates that arise during the mokA-mediated biosynthesis of the nonaketide chain and leads to dihydromonacolin L (PubMed:19693441). Covalently bound dihydromonacolin L is released from mokA by the mokD esterase (By similarity). Conversion of dihydromonacolin L into monacolin L and then monacolin J is subsequently performed with the participation of molecular oxygen and P450 monoogygenase mokC (PubMed:19693441). Finally, mokF performs the conversion of monacoline J to monacoline K through the addition of the side-chain diketide moiety (2R)-2-methylbutanoate produced by the diketide synthase mokB (PubMed:19693441). HMG-CoA reductase mokG may act as a down-regulator of monacolin K production (PubMed:18578535).
Mass
49.306 kDa
Sequence
MALSPVQDPPSHTDKTMPRRAFRRSCDRCHAQKIKCIGSEGAVARASCQRCQQAGLRCVYSERCPKRKLPKPNPAESSPASSTAGLHTSSSDSSPPVPSDGLPLDLPGPDSSGVSLQFLDPSADCDWPWSSIGVDETVVNNCLDLSHGHGHGDLSCQLELPMPDLPSPFEFSAEKSPSPSVSGSIAGAVSAQRELFDGLSTVSQELEAILLAVAVEWPKQEIWTYPIGTFFNASRRLLVYLQQQSNTRSDQGMLNECLRTKNLFMAVHCYMLIVKIFTSLSELLLSQIRHSQAGQLTPLEGHQFEPPPSSSRDRSSVDTMPIFNPNLHIGGLFSYLNPFMHALSSACTTLRVGVQLLRENESALGIPPAQGVAASVSMGKEEWADGEDVASAVTTADEDLRQPASRILSMVWSDEVGDQKAKSADAAGPRSRTLAVLRRCNREIFSLARQHNLAS