Protein
4-hydroxyphenylacetate 3-monooxygenase reductase component
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function
Reductase component of the 4-hydroxyphenylacetate (HPA) 3-hydroxylase. Catalyzes the reduction of FAD by NADH. The reduced flavin is then transferred to the oxygenase component HpaB. Is also able to reduce FMN and riboflavin, but preferentially binds FAD. Has no activity with NADPH as the reductant.
Similarity
Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily.
Sequence
MSQLEPRQQAFRNAMAHLSAAVNVITSNGPAGRCGITATAVCSVTDSPPTLMLCINRNSEMNTVFKANGRLCVNVLSGEHEEVARHFAGMTEVPMERRFALHDWREGLAGLPVLHGALANLQGRIAEVQEIGTHSVLLLELEDIQVLEQGDGLVYFSRSFHRLQCPRRAA