gtf3

Gene

gtf3

Protein

N-acetylglucosaminyltransferase

Organism

Lactobacillus reuteri (strain ATCC 53608)

Length

340 amino acids

Function

Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall protein(s) encoded upstream in the same operon.

Similarity

Belongs to the Gtf3 glucosyltransferase family.

Mass

38.767 kDa

Sequence

MEGLSLTVHITNLYGQSFQSTAQIAQNQIAKIGRELGFNELGIYNYNWPDEPSVALDTRFDGIIASVSNNDTVIFQSPTWNSIEWDQAFIDHLAPYNVKKIIFIHDIIPLMFESNRYLLPQFIDYYNKADLIIAPSQPMVDFLRANGLTVEKVVLQHMWDHCASVDFTVTLQNTGVINFAGNLEKFQLVGHWHYPDNPLYAFAKVIDVEPTDNIKFMGWQSDPVLLSKLRHNGGFGLVWSNEPYCKNYMHLNANHKLSTYLAAGLPVIVNENIAESETILRKGLGIVADNLDEAIEKVQGMDDQSYNEMVQRVDDFARLIREGYFAKKALTEAVFKLYYQ

Protein

N-acetylglucosaminyltransferase: gtf3

Gene

gtf3

Protein

Glucosyltransferase 3

Organism

Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Length

336 amino acids

Function

Required for polymorphic O-glycosylation of the serine-rich repeat protein PsrP. Catalyzes the second step in glycosylation of the serine-rich repeat protein in this bacteria. Transfers glucose from UDP-glucose to the terminal GlcNAc moiety of 3-O-(N-acetyl-alpha-D-glucosaminyl)-L-seryl-[protein] which results from the first glycosylation step of PsrP (using the short substrate SRR1-GlcNAc) (PubMed:28246170, PubMed:25404702, PubMed:21653318). Has weak hydrolytic activity against UDP-glucose and even weaker activity on UDP-galactose (PubMed:28246170). Does not complement deletion of the gtf3 gene from S.parasanguinis strain FW213 (PubMed:21653318).

Similarity

Belongs to the Gtf3 glucosyltransferase family.

Mass

38.368 kDa

Sequence

MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIASDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDVVPLMFDSNYYLMKDYLYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDLSLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARSLSIEGWKKDEELLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIVDQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLGID

Protein

Glucosyltransferase 3: gtf3

Gene

gtf3

Protein

Glucosyltransferase 3

Organism

Lactobacillus reuteri (strain DSM 17509 / CIP 109821 / 100-23)

Length

334 amino acids

Function

Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall protein(s) encoded upstream in the same operon.

Similarity

Belongs to the Gtf3 glucosyltransferase family.

Mass

38.194 kDa

Sequence

MTVHITNLYGQSFQSTAQIAQNQIAKIGRELGFNELGIYSYNWPDEPSVALDTRFDGIIASVSNNDTVIFQSPTWNSIEWDQAFIDHLAPYNVKKIIFIHDIIPLMFESNRYLLPQFIDYYNKADLIIAPSQPMVDFLRANGLTVEKVVLQHMWDHYASVDFTVTPQNTGVINLAGNLEKFQLVGHWHYPNNPLYAFAKVIDIEPTDNIKFMGWQSDPVLLSKLRHNGGFGLVWSNESYWKNYMHLNANHKLSTYLAAGLPVIVNENIAESETILRKGLGIVADNLDEAIEKVQGMDDQSYNEMVQRVDDFARLIREGYFAKKALTEAVFNLYY

Protein

Glucosyltransferase 3: gtf3

Gene

gtf3

Protein

Glucosyltransferase 3

Organism

Streptococcus gordonii

Length

334 amino acids

Function

Part of the accessory SecA2/SecY2 system specifically required to export GspB, a serine-rich repeat cell wall protein encoded upstream in the same operon.

Similarity

Belongs to the Gtf3 glucosyltransferase family.

Mass

38.007 kDa

Sequence

MKVNITNLYGMSGQSTALIAQNDVTKLAKQLGFNELSFYFYDIYSDSQSELSRRLDGIMASVGYGDVVIYQSPTWNGREFDQAFISKLKILQAKLITFIHDVPPLMFPSNYYLMPEYIDMYNQSDAVIVPSEQMRDKLLAEGLTVNKILIQRMWDHPYDLPLHQPQFAPKLYFAGSVERFPHLINWSYATPLEIFSPEEESNPEANVSYCGWVSRPELLLELSKGGLGLVWGVEDNPADEPEYYGLNISHKSATYLAAGIPVIVPSYLSNAELIRERGLGFVVDSLEEASRIVENLTVEEYQDMVERVRKFSFLLKEGYFSKKVLIDAVMEVLS

Protein

Glucosyltransferase 3: gtf3

Gene

gtf3

Protein

Glucosyltransferase 3

Organism

Streptococcus agalactiae serotype III (strain COH1)

Length

331 amino acids

Function

Required for polymorphic O-glycosylation of the serine-rich repeat protein Srr2. Catalyzes the second step in glycosylation of the serine-rich repeat protein in this bacteria. Transfers glucose from UDP-glucose to the terminal GlcNAc moiety of 3-O-(N-acetyl-alpha-D-glucosaminyl)-L-seryl-[protein] which results from the first glycosylation step of Srr2; does not use other sugar nucleotides as substrates (PubMed:25404702, PubMed:21653318). Complements deletion of the gtf3 gene from S.parasanguinis strain FW213 (PubMed:21653318).

Similarity

Belongs to the Gtf3 glucosyltransferase family.

Mass

37.963 kDa

Sequence

MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPIDTDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDVVPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNITLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLIMKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGFIIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM

Protein

Glucosyltransferase 3: gtf3

Gene

gtf3

Protein

Glucosyltransferase 3

Organism

Streptococcus parasanguinis

Length

330 amino acids

Function

Required for polymorphic O-glycosylation of the serine-rich repeat protein Fap1. Catalyzes the second step in glycosylation of the serine-rich repeat protein in this bacteria. Transfers glucose from UDP-glucose to the terminal GlcNAc moiety of 3-O-(N-acetyl-alpha-D-glucosaminyl)-L-seryl-[protein] which results from the first glycosylation step of Fap1; does not use other sugar nucleotides as substrates.

Similarity

Belongs to the Gtf3 glucosyltransferase family.

Mass

38.053 kDa

Sequence

MRVYITNINGQSIQSTAQLCQNTVTDVAVSLGYRELGIYCYQIHTDSESELSKRLDGIVAGLRHGDVVIFQTPTWNTTEFDEKLMNKLKLYDIKIVLFIHDVVPLMFSGNFYLMDRTIAYYNKADVVVAPSQKMIDKLRDFGMNVSKTVVQGMWDHPTQAPMFPAGLKREIHFPGNPERFSFVKEWKYDIPLKVYTWQNVELPQNVHKINYRPDEQLLMEMSQGGFGLVWMDDKDKEYQSLYCSYKLGSFLAAGIPVIVQEGIANQELIENNGLGWIVKDVEEAIMKVKNVNEDEYIELVKNVRSFNPILRKGFFTRRLLTESVFQAICD

Protein

Glucosyltransferase 3: gtf3