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Protein expression services for gtf3 | Glucosyltransferase 3

Description
Required for polymorphic O-glycosylation of the serine-rich repeat protein PsrP. Catalyzes the second step in glycosylation of the serine-rich repeat protein in this bacteria. Transfers glucose from UDP-glucose to the terminal GlcNAc moiety of 3-O-(N-acetyl-alpha-D-glucosaminyl)-L-seryl-[protein] which results from the first glycosylation step of PsrP (using the short substrate SRR1-GlcNAc) (PubMed:28246170, PubMed:25404702, PubMed:21653318). Has weak hydrolytic activity against UDP-glucose and even weaker activity on UDP-galactose (PubMed:28246170). Does not complement deletion of the gtf3 gene from S.parasanguinis strain FW213 (PubMed:21653318).
Family
Belongs to the Gtf3 glucosyltransferase family.
Species
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Length
336 amino acids
Sequence
MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIASDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDVVPLMFDSNYYLMKDYLYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDLSLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARSLSIEGWKKDEELLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIVDQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLGID
Mass
38.4 kDa
Simulated SDS-PAGE
Western blot of gtf3 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make gtf3 using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

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