Description
Required for polymorphic O-glycosylation of the serine-rich repeat protein PsrP. Catalyzes the second step in glycosylation of the serine-rich repeat protein in this bacteria. Transfers glucose from UDP-glucose to the terminal GlcNAc moiety of 3-O-(N-acetyl-alpha-D-glucosaminyl)-L-seryl-[protein] which results from the first glycosylation step of PsrP (using the short substrate SRR1-GlcNAc) (PubMed:28246170, PubMed:25404702, PubMed:21653318). Has weak hydrolytic activity against UDP-glucose and even weaker activity on UDP-galactose (PubMed:28246170). Does not complement deletion of the gtf3 gene from S.parasanguinis strain FW213 (PubMed:21653318).
Family
Belongs to the Gtf3 glucosyltransferase family.
Species
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Sequence
MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIASDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDVVPLMFDSNYYLMKDYLYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDLSLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARSLSIEGWKKDEELLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIVDQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLGID
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service