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chlF

Gene
chlF
Protein
Light-dependent chlorophyll f synthase
Organism
Synechococcus sp. (strain ATCC 29403 / PCC 7335)
Length
419 amino acids
Function
Synthesizes chlorophyll f or chlorophyllide f (Chl f, 2-formyl chlorophyll a), probably by oxidation of chlorophyll a or chlorophyllide a and reduction of plastoquinone. The reaction is probably light-dependent. Chl f absorbs far red light (FRL, 707 nm in 100% methanol), and is synthesized when cells are grown in FRL, where it provides the advantage of extending the spectral range of harvested light in terrestrial cyanobacteria (PubMed:27386923). Chl f synthesis is probably light-dependent (PubMed:27386923).
Similarity
Belongs to the reaction center PufL/M/PsbA/D family.
Mass
45.268 kDa
Sequence
MIQTGFGRTSALEGFEQPFDPAQAIDLESPLTSTDTSVENTTRNAGALWPSSQPLSPWERFCRWVTSTENRIYIGWFGMLAIPTLATAAIVFVLAIIAAPAVDMDGTGRMVSGSLLDGNNLITAAVVPTSAAIGLHFYPIWEAASLDEWLINGGPYQLIVLHFIIGIISYQDREWELSYRLKMRPWISLAFTAPVAASVSVLLVYPVGQGGFASGMPLGISGTFTFMMQFQADHNILASPLHQMGVIGVLGGALLCAVHGSLVTSTVCRAPAQTMALTTTKTGTDRQKPKKAKTYSFEHAQAYQQTLLWRGAKFNSSRAVHFCLAALPVAGIWSAAIGVDLAAFDFDRLSFELPSHISVRKTVVPTWSDVVNQANLGIHTVGEKTPPKFSESGFPEFKLSEFVEPIAEDSASTLLSPHS

Gene
chlF
Protein
Light-dependent chlorophyll f synthase
Organism
Chlorogloeopsis fritschii (strain PCC 9212)
Length
376 amino acids
Function
Synthesizes chlorophyll f or chlorophyllide f (Chl f, 2-formyl chlorophyll a), probably by oxidation of chlorophyll a or chlorophyllide a and reduction of plastoquinone. The reaction is probably light-dependent (PubMed:27386923). Chl f absorbs far red light (FRL, 707 nm in 100% methanol), and is synthesized when cells are grown in FRL, where it provides the advantage of extending the spectral range of harvested light in terrestrial cyanobacteria (PubMed:27386923). When ectopically expressed in Synechococcus PCC 7002 (which does not grow in FRL and does not make Chl f) produces Chl f (0.059% of total chlorophyll) (PubMed:27386923).
Similarity
Belongs to the reaction center PufL/M/PsbA/D family.
Mass
41.66 kDa
Sequence
MKLESDHVIATSDSSDYTSEPTANKLSKRRKKVNYWEKFCSWVTSTENRLYVGWFGVLMIPCVLTAATVFIIAIIAAPPVDMDGIGVPISGSILSGNNIITAAVVPTSAAIGLHFYPIWEAASIDEWLYNGGPYQLIVLHFLIGIIAYQDREWELSYRLGMRPWISLAFTAPVAASVSVLLIYPVGQGSLSAGMPLGISGTFHFMLQFQADHNILMSPLHQLGVIGVLGGAFAAAMHGSLVTSTLIRSHNHSESESINKGYKLGQQHPTYNFRSAQVYLWHLIWQRVSFPNSRKLHFFLAALPVAGIWSAALGVDIAAFDFDYLQFHQPELKSQGQIIHTWADTIDWASLGIKVLDERHIYDFPENLTAGEVVPWK