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Protein expression services for chlF | Light-dependent chlorophyll f synthase

Description
Synthesizes chlorophyll f or chlorophyllide f (Chl f, 2-formyl chlorophyll a), probably by oxidation of chlorophyll a or chlorophyllide a and reduction of plastoquinone. The reaction is probably light-dependent (PubMed:27386923). Chl f absorbs far red light (FRL, 707 nm in 100% methanol), and is synthesized when cells are grown in FRL, where it provides the advantage of extending the spectral range of harvested light in terrestrial cyanobacteria (PubMed:27386923). When ectopically expressed in Synechococcus PCC 7002 (which does not grow in FRL and does not make Chl f) produces Chl f (0.059% of total chlorophyll) (PubMed:27386923).
Family
Belongs to the reaction center PufL/M/PsbA/D family.
Species
Chlorogloeopsis fritschii (strain PCC 9212)
Length
376 amino acids
Sequence
MKLESDHVIATSDSSDYTSEPTANKLSKRRKKVNYWEKFCSWVTSTENRLYVGWFGVLMIPCVLTAATVFIIAIIAAPPVDMDGIGVPISGSILSGNNIITAAVVPTSAAIGLHFYPIWEAASIDEWLYNGGPYQLIVLHFLIGIIAYQDREWELSYRLGMRPWISLAFTAPVAASVSVLLIYPVGQGSLSAGMPLGISGTFHFMLQFQADHNILMSPLHQLGVIGVLGGAFAAAMHGSLVTSTLIRSHNHSESESINKGYKLGQQHPTYNFRSAQVYLWHLIWQRVSFPNSRKLHFFLAALPVAGIWSAALGVDIAAFDFDYLQFHQPELKSQGQIIHTWADTIDWASLGIKVLDERHIYDFPENLTAGEVVPWK
Mass
41.7 kDa
Simulated SDS-PAGE
Western blot of chlF recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make chlF using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

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