Protein
dTDP-glucose 4,6-dehydratase
Organism
Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110)
Function
Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose (PubMed:10196166). Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity).
Similarity
Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.
Sequence
MKILVTGGAGFIGSHFVTSLISGDIATPQPVTQVTVVDKLGYGGNLRNLAEASADPRFSFVRGDICDEGLIEGLMARHDTVAHFAAETHVDRSVVASGPFVASNLVGTQVLLDAALRHHIGRFLHVSTDEVYGSIDTGSWAEGHPLAPNSPYAASKAGSDLLALAYHQTHGMDVVVTRCSNNYGPRQFPEKMIPLFVTRLLDGLDVPVYGDGRNIRDWLHVSDHCRGLALALGAGRAGEVYHIGGGWEATNLELTEILLEACGAPASRISFVTDRKGHDRRYSLDYSKIAGELGYRPRVDFTDGIAETVAWYRANRSWWT