Protein
Enolase-phosphatase E1
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Similarity
Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.
Sequence
MTIKAILTDIEGTTSAVSFVFDVLFPYAARHLPDFVREHAGETEVAAQLAAVRAESGEADADVERVIAILLQWIAEDRKATPLKALQGMVWAQGYRDGQLKGHVYPDAVQALREWKARGLDLYVYSSGSIQAQKLIFGCSEAGDLGSLFSGYFDTTSGPKRESASYARIAGAIGLPAAEILFLSDVVQELDAARDAGMRTLGLAREGGSLDGHPTVASSPTSSWSERAGYPEGTLLLGSIAPWVPPSAG