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AF_1318

Gene
AF_1318
Protein
Piwi protein AF_1318
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Length
427 amino acids
Function
Might play a role in defense against invading genetic elements, using short DNA sequences as guides to bind complementary target strands, resulting in slicing of the target nucleic acid (By similarity). Binds nucleic acids with decreasing affinity in the following order; ssDNA, ssRNA, dsDNA, RNA-DNA, RNA-RNA (PubMed:15800629). Association of the 5' seed region of the guide strand (nucleotides 2-7) with AfPiwi increases affinity for the corresponding target strand; the greatest increase in affinity is for guide DNA with target RNA (PubMed:19187762).
Similarity
Belongs to the argonaute family. Short pAgo subfamily.
Mass
49.244 kDa
Sequence
MMEYKIVENGLTYRIGNGASVPISNTGELIKGLRNYGPYEVPSLKYNQIALIHNNQFSSLINQLKSQISSKIDEVWHIHNINISEFIYDSPHFDSIKSQVDNAIDTGVDGIMLVLPEYNTPLYYKLKSYLINSIPSQFMRYDILSNRNLTFYVDNLLVQFVSKLGGKPWILNVDPEKGSDIIIGTGATRIDNVNLFCFAMVFKKDGTMLWNEISPIVTSSEYLTYLKSTIKKVVYGFKKSNPDWDVEKLTLHVSGKRPKMKDGETKILKETVEELKKQEMVSRDVKYAILHLNETHPFWVMGDPNNRFHPYEGTKVKLSSKRYLLTLLQPYLKRNGLEMVTPIKPLSVEIVSDNWTSEEYYHNVHEILDEIYYLSKMNWRGFRSRNLPVTVNYPKLVAGIIANVNRYGGYPINPEGNRSLQTNPWFL