Required for rescue of stalled ribosomes mediated by trans-translation (By similarity). Binds to and required for stability of mature tmRNA, which it protects from degradation by RNase R (rnr) (PubMed:12511504). Required for correct localization of tmRNA (PubMed:19805312). In this organism the ssrA gene for tmRNA is circularly permutated and is processed into 2 pieces to function (PubMed:10884408). Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.

Belongs to the SmpB family.

152 amino acids

MSKPIAENRRARFDYFIEETFEAGIMLTGTEVKSLRTGRANIAESYASVEGREIVLINADIPPYGHANRFNHEPRRHRKLLLHRRQIDKLIGAVQREGRTLVPIKLYWNDKGLAKLEVGLAKGKKLHDKRDTAAERDWQRDKARLMKGDRGD

17.5 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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