Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity. In pM quantities promotes the resuscitation and growth of dormant, nongrowing cells from M.luteus in addition to Mycobacterium tuberculosis, M.avium, M.bovis, M.kansaii and M.smegmatis. Hydrolyzes endogeneous cell walls, peptidoglycan preparations from Mycobacterium tuberculosis and M.smegmatis as well as an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG). Overexpression in E.coli (when the enzyme is targeted to the periplasm) causes cell lysis.

Belongs to the transglycosylase family. Rpf subfamily.

223 amino acids

MDTMTLFTTSATRSRRATASIVAGMTLAGAAAVGFSAPAQAATVDTWDRLAECESNGTWDINTGNGFYGGVQFTLSSWQAVGGEGYPHQASKAEQIKRAEILQDLQGWGAWPLCSQKLGLTQADADAGDVDATEAAPVAVERTATVQRQSAADEAAAEQAAAAEQAVVAEAETIVVKSGDSLWTLANEYEVEGGWTALYEANKGAVSDAAVIYVGQELVLPQA

23.2 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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