A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.

Belongs to the helicase family. UvrD subfamily.

1169 amino acids

MNKILEKIQNNTTILIEASAGTGKTHILENVVINLIKTKLYSINEILVLTFTKKATEEMHTRILKVIENAYSNSKTNEILKEAYEQSKKLFISTINKFALHALNNFQIETENYSKYKPKEKFSKEIDEIVYDFLRKSDSLIQALDIKDYELKVFKSDAKKTEEIVLKIKKAYERDTTQELGDWLKTQTAFENILLKKEELIKDYNKIIEDLDKMTKDEILSFYNKHIQTGKLEIEYSKENDIFKIAETLLKNKFFSTLIEKETKKNSKLSPKELKIKNDLICLGINIKHEKYKSEDNRNKNRNNLKQYVILKVEYKILKYIEKELKKTIKSTNTIDQNYIISNLKNYLKSEDKKLLNAIKNRYKIILIDEAQDLSLIQIEIFKILKTAGIKLIFIADPKQIIYSFRKADISFYNKEIKNKINTDARIVLKINHRSSKKLIGPLNKIFNNIYNNAIADEIEKIDFTNSLPNQKNDNNKIVINGQEIEGINIITTNTESEEDIYQKTALTIKYLLAYGKIAENNKIRNIKMQDIKVLCRGKNEINLIDKALKKEQIQTNKTQEKFLKTKEFSEIFYIIKCLDRKQSFKTLNYILSSKILNVPWNLQRILIKQDKICLIEEFIENIIVLLEKNEITLINAINKITFEKNLWIKIANITKDQKIIEWAKNKINYKGLLIKEGKLENLKTYETTLEIISKIYHKEQNIQSLISTLESLIINEEPEEIEEKINNINNDNESIELMTIHKSKGLGMNIVFLLNTTPIENSNFFSKKNQFYKFYQDGKIEYDFFKLEENKKYARLKILSEEKNIFYVGATRAKFALFIIKINSITSKLLEIAKIFTIDDIKHDFNIHEFIGQKRFNKKKYNTNVNTKLIPPKPIIKNMFKKEYTSSFSSLTAQAHHKEFYENYDFKNINYEKETELDYEPGLEETLPKGKDIGNILHAAMEEIIFSTAKDTFDNFKKNNIEIIEKQIQKINSNLNTIEIQNSLAKMIYNILTYNIRAINTRLCDIEELQKEMEFLIKINPEFQKQKYLFDKHFEDLHIKLSDGYLKGIVDLIFKANNKIYILDYKTNYLGKNKEDYNITNLENTIKKEYYDLQYKIYALGIKKILFKNKKEYNQKFGGIIYLFTRAFEDNIECLKSKFENGIYFNLPKFNDVDLDKIILELGIKRHL

137.8 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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