Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction.

Belongs to the NAD-dependent DNA ligase family.

615 amino acids

MDETQQLLYKFKNLTISENEKLKLKEKADHFYFNTEKEILTDNEYDILAEQLQSTNVGCSPLISKTDLPLWMGSLDKVYNDKELNLWIKKVASDKYIIQCKLDGVSCLIINKDNKLKAYTRGNGKVGTDISHLIKYFIKDKCLPNNIALRCEIIIKKETFNQKYKHAFSNPRSFVSGVVNRKQENIVISELNDLCLIAYELINFPVNEYQKNILTQIEIIEQLNFIQFVNFKTISNEFLTQKALSNLFKEMKNNSLFEMDGLVILANTPYIRVTEGNPKHSIAFKVRGDNVKEAKVTFIEWNVGKTGVFTPKVHIVPTEINGTTVSCFTGFNANYLIEKGIGEGAIILVTRAGDAIPQIIGVKQTGVLTFPKDYEWKGDCRIVEKIESKERIIKQILHFVESVDIPYIKEATINKLYNNGCTSIELFLKLTQKDLLLFGPKLSSTIYNSIQKSFEAPIEKFLSGYNAFGDYIGEKKILLLLQKYPNLFELENLDAIDLVSIEGIGSKTATQIKQHFINAKLIYNNIIQNKLFKGVLHTPSKQNTVELFKVCVSGTRDPLFIQELKNRGFVLSDNITKKVKVLIVKNSNEETTKVKKANSVGITILTLEDFKQKYF

70.2 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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