Try expressing rad25 in cell-free | Putative DNA helicase Rad25
Family
Belongs to the helicase family. Archaeal Rad25 subfamily.
Species
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Length
621 amino acids
Sequence
MTDEEPADRDDDSDSTPELELDAVYDAIDAVGRPHLTATEFSRKTDLTPDEARAALERLADDGDIERQDVSEVESVWYPTDIAEVTDRERVVLFPDRREVVVEHPDQFTRAQLSQFARLQDTNRSGGYVYELREEDIWAAPHESLDDLLTTMRDVLGERSPHLEEWVTSQWERARKFRLKTHEDGYVVLEAESDDLMGNVARPKLDDDHLRAPISDSESWVNEDATAEIKRTLYEAGYPVRDDRDLETGDAIEMDLRLRLRDYQQDWVERFTEQGSGVFVGPPGSGKTVAAMGAMAAIGGETLILVPSRELATQWRDELVRHTSLTDDDIGEYHGGEKEIRAVTIATYRTAGMDRHRKLFDQRKWGLIVFDEVHHVPSPIHRRSADLQTKHRLGLTATPTRESDDEEEIFTLIGPPIGTDWGKLFDEGYVAEPEVEIRLVPWGDETEQSEYSSTSGHDRRQAAASNTGKIDEIRYALAENPAAKALVFIEYLDQGEAISEAIDAPFISGETPHARREKLFDEFRRGELTTLVVSRVGDEGIDLPDAELALVASGLGGSRRQGAQRAGRTMRPAGDARMVILATRGTTEEDFVRRQMRHLASKGIRVTETEAEAVEPPAKTE
Mass
69.9 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
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Cell-Free protein synthesis
Have you tried producing rad25 in a cell-free protein expression systems? We have solved cell-free protein expression scale-up and purification challenges so that you can obtain up to low-milligram quantities of proteins in hours.
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