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Pou3f2 expression plasmid | POU domain, class 3, transcription factor 2

Description
Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (PubMed:8276233). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).
Family
Belongs to the POU transcription factor family. Class-3 subfamily.
Species
Rattus norvegicus
Length
445 amino acids
Sequence
MATAASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLQVQGDYGALQSNGHPLSHAHWITALSHGGSGGGGGGGGGGGGGGGGGGDGSPWSTSPLGQPDIKPSVVVQQGGRGDELHGPGALQQQHQQQQQQQQQQQQQQQQQQQQQQQRPPHLVHHAANNHPGPGAWRSAAAAAHLPPSMGASNGGLLYSQPSFTVNGMLGAGGQPAGLHHHGLRDAHDEPHHADHHPHPHSHPHQQPPPPPPPQGPPGHPGAHHDPHSDEDTPTSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTSIDKIASQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGGTLPGAEDVYGGSRDTPPHHGVQTPVQ
Mass
47.2 kDa
SDS-PAGE
Western blot of Pou3f2 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Purity
Purified using anion exchange chromatography.
Expression
E. Coli Cell-Free System. We recommend using Synbio Studio's cell-free proteine expression reagents.
Format
Predominantly supercoiled plasmid.
Tags
Custom tags are available upon request.
Sterility
Sterile filtering available upon request.
Endotoxin
Low endotoxin available upon request.
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.