About Products Protein Database Contact

Protein expression services for ptaJ | Oxidoreductase ptaJ

Description
Oxidoreductase; part of the gene cluster that mediates the biosynthesis of pestheic acid, a diphenyl ether which is a biosynthetic precursor of the unique chloropupukeananes (PubMed:24302702). The biosynthesis initiates from condensation of acetate and malonate units catalyzed by the non-reducing PKS ptaA (PubMed:24302702). As the ptaA protein is TE/CLC domain-deficient, hydrolysis and Claisen cyclization of the polyketide could be catalyzed by ptaB containing a beta-lactamase domain (PubMed:24302702). The ptaB protein might hydrolyze the thioester bond between the ACP of ptaA and the intermediate to release atrochrysone carboxylic acid, which is spontaneously dehydrated to form endocrocin anthrone (PubMed:24302702). Endocrocin anthrone is then converted to endocrocin, catalyzed by the anthrone oxygenase ptaC (PubMed:24302702). Spontaneous decarboxylation of endocrocin occurs to generate emodin (PubMed:24302702). An O-methyltransferase (ptaH or ptaI) could methylate emodin to form physcion (PubMed:24302702). PtaJ could then catalyze the oxidative cleavage of physcion, and rotation of the intermediate could then afford desmethylisosulochrin (PubMed:24302702). PtaF, a putative NADH-dependent oxidoreductase, might also participate in the oxidative cleavage step (PubMed:24302702). Desmethylisosulochrin is then transformed by another O-methyltransferase (ptaH or ptaJ) to form isosulochrin (PubMed:24302702). Chlorination of isosulochrin by ptaM in the cyclohexadienone B ring then produces chloroisosulochrin (PubMed:24302702). PtaE is responsible for the oxidative coupling reactions of both benzophenones isosulouchrin and chloroisosulouchrin to RES-1214-1 and pestheic acid respectively, regardless of chlorination.
Family
Belongs to the questin oxidase family.
Species
Pestalotiopsis fici (strain W106-1 / CGMCC3.15140)
Length
444 amino acids
Sequence
MAASTAAQVQLSEEALGLARIFENPKGSLEAASKLLQKNHDEFHVFWRDVGGHNHIPHSVLSILALGGGPAELQRAWDDGVAIQRPTPPLDEDVVKKLENPAEFRARIGSIPNYTNFLHFFRNQMDKKGWQAVVSEYAFSRTPLAETIFAQLFEGAYHPFIHIGFGIEFNLPSIIAEGLAQAATHDSAGIEGFFLEAERQAAQSKGPGKSLVQLLDEVRTTEKIKTAARLPDGPVRVRDGVIGRAGAEIAALASQFRVPADQLSRGAAESINISAYTAGAAQRAGKARKIDFFHMHNTTSSLFLTVFLNQPWISTEDKVRIVEWKGRLDLVWYAACSAPDLNVDHVIGYKPAQSAGWGWKELYEAINVAHDDGHLAKIVRALKNGEEVSRPFESGEGAEAFPIKGDSWLKLAQMSYDTTLDLPDDDKWIWGAGFLPLWNKVPSL
Mass
48.8 kDa
Simulated SDS-PAGE
Western blot of ptaJ recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make ptaJ using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

Order Here