Protein expression services for pikAIII | Narbonolide/10-deoxymethynolide synthase PikA3, module 5
Description
Involved in the biosynthesis of 12- and 14-membered ring macrolactone antibiotics such as methymycin and neomethymycin, and pikromycin and narbomycin, respectively. Component of the pikromycin PKS which catalyzes the biosynthesis of both precursors 10-deoxymethynolide (12-membered ring macrolactone) and narbonolide (14-membered ring macrolactone). Chain elongation through PikAI, PikAII and PikAIII followed by thioesterase catalyzed termination results in the production of 10-deoxymethynolide, while continued elongation through PikAIV, followed by thioesterase (TE) catalyzed cyclization results in the biosynthesis of the narbonolide.
Species
Streptomyces venezuelae
Length
1562 amino acids
Sequence
MANNEDKLRDYLKRVTAELQQNTRRLREIEGRTHEPVAIVGMACRLPGGVASPEDLWQLVAGDGDAISEFPQDRGWDVEGLYDPDPDASGRTYCRSGGFLHDAGEFDADFFGISPREALAMDPQQRLSLTTAWEAIESAGIDPTALKGSGLGVFVGGWHTGYTSGQTTAVQSPELEGHLVSGAALGFLSGRIAYVLGTDGPALTVDTACSSSLVALHLAVQALRKGECDMALAGGVTVMPNADLFVQFSRQRGLAADGRSKAFATSADGFGPAEGAGVLLVERLSDARRNGHRILAVVRGSAVNQDGASNGLTAPHGPSQQRVIRRALADARLAPGDVDVVEAHGTGTRLGDPIEAQALIATYGQEKSSEQPLRLGALKSNIGHTQAAAGVAGVIKMVQAMRHGLLPKTLHVDEPSDQIDWSAGTVELLTEAVDWPEKQDGGLRRAAVSSFGISGTNAHVVLEEAPAVEDSPAVEPPAGGGVVPWPVSAKTPAALDAQIGQLAAYADGRTDVDPAVAARALVDSRTAMEHRAVAVGDSREALRDALRMPEGLVRGTSSDVGRVAFVFPGQGTQWAGMGAELLDSSPEFAASMAECETALSRYVDWSLEAVVRQEPGAPTLDRVDVVQPVTFAVMVSLAKVWQHHGITPQAVVGHSQGEIAAAYVAGALTLDDAARVVTLRSKSIAAHLAGKGGMISLALDEAAVLKRLSDFDGLSVAAVNGPTATVVSGDPTQIEELARTCEADGVRARIIPVDYASHSRQVEIIEKELAEVLAGLAPQAPHVPFFSTLEGTWITEPVLDGTYWYRNLRHRVGFAPAVETLAVDGFTHFIEVSAHPVLTMTLPETVTGLGTLRREQGGQERLVTSLAEAWANGLTIDWAPILPTATGHHPELPTYAFQTERFWLQSSAPTSAADDWRYRVEWKPLTASGQADLSGRWIVAVGSEPEAELLGALKAAGAEVDVLEAGADDDREALAARLTALTTGDGFTGVVSLLDDLVPQVAWVQALGDAGIKAPLWSVTQGAVSVGRLDTPADPDRAMLWGLGRVVALEHPERWAGLVDLPAQPDAAALAHLVTALSGATGEDQIAIRTTGLHARRLARAPLHGRRPTRDWQPHGTVLITGGTGALGSHAARWMAHHGAEHLLLVSRSGEQAPGATQLTAELTASGARVTIAACDVADPHAMRTLLDAIPAETPLTAVVHTAGAPGGDPLDVTGPEDIARILGAKTSGAEVLDDLLRGTPLDAFVLYSSNAGVWGSGSQGVYAAANAHLDALAARRRARGETATSVAWGLWAGDGMGRGADDAYWQRRGIRPMSPDRALDELAKALSHDETFVAVADVDWERFAPAFTVSRPSLLLDGVPEARQALAAPVGAPAPGDAAVAPTGQSSALAAITALPEPERRPALLTLVRTHAAAVLGHSSPDRVAPGRAFTELGFDSLTAVQLRNQLSTVVGNRLPATTVFDHPTPAALAAHLHEAYLAPAEPAPTDWEGRVRRALAELPLDRLRDAGVLDTVLRLTGIEPEPGSGGSDGGAADPGAEPEASIDDLDAEALIRMALGPRNT
Mass
163.6 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
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Protein synthesis service
Make pikAIII using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)
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