Catalyzes the conversion of monodehydroascorbate (MDA) to ascorbate, oxidizing NADH in the process. Can also use 2,4,6-trinitrotoluene (TNT) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates, but not 1-chloro-4-nitrobenzene (CNB).

Belongs to the FAD-dependent oxidoreductase family.

493 amino acids

MSAVRRVMALASTTLPTKSGLSLWCPSSPSLARRFPARFSPIGSRIASRSLVTASFANENREFVIVGGGNAAGYAARTFVENGMADGRLCIVTKEAYAPYERPALTKAYLFPPEKKPARLPGFHTCVGGGGERQTPDWYKEKGIEVIYEDPVAGADFEKQTLTTDAGKQLKYGSLIIATGCTASRFPDKIGGHLPGVHYIREVADADSLIASLGKAKKIVIVGGGYIGMEVAAAAVAWNLDTTIVFPEDQLLQRLFTPSLAQKYEELYRQNGVKFVKGASINNLEAGSDGRVSAVKLADGSTIEADTVVIGIGAKPAIGPFETLAMNKSIGGIQVDGLFRTSTPGIFAIGDVAAFPLKIYDRMTRVEHVDHARRSAQHCVKSLLTAHTDTYDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETVEVGNFDPKIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSVEEALEIAQAALQS

53.3 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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