This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SinI endonuclease.

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

461 amino acids

MIMNDIITVTEAAQLLELTPQRVRTMCKQGSIDAYQSGRTWLIKSSSVEKLMLVNSLSDAQNSYSMLASEPKNKPKALSFFSGAMGLDLGIEQAGFETLLASEIDKAARDTILSNRPNMALIGDIRDYTTEDILKLAGVSSGNEIDLIMGGPPCQAFSTAGKRLGLEDERGNVFIKYLDVALDIRPKYIVIENVRGLLSAPMKHRPHNERGEGLPPLKSEEQPGGVLHYIIRIIKSAGYSVSFNLYNSANFGVPQIRERVIIICSRDGSRVPFLQPTHSEKGEYGLPKWITLRETITNLKNITHEHVLFPEKRLKYYRLLKEGQYWKHLPEDLQKEALGKSFFLGGGKTGFLRRVAWDRPSPTLVTHPAMPATDLAHPDELRPLSVQEYKVIQQFPEEWVIKGKLLDKYRQLGNAVPIGLGLAVGKNILDHMNGRKIESFPNFRYSRYKNTSDLDIFGELV

51.8 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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