This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MthZI endonuclease.

Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

355 amino acids

MKTTHRIYFKNSADMNELKDKSINLVVTSPPYPMVEIWDRLFSELNPKIEETLIDEEDGLRSYNLMHEELEKVWHEVDRVTAPGGVVIINIGDATRKIGKKFQLYPNHVRTIDFFFDRGYQVLPFIIWRKQSNKPTKFMGSGMLPPNAYVTHEHEYILIFRKEGPRQFKTEEERKLRRESAYFWEERNQWFSDVWTDLTGVSQRLNHKNLRKRAAAYPFELAYRLINMYSIMGDWVLDPFLGTGTTMIAAACAGRNSIGYELDHNFKDLIESRINETLKLSNEIVMRRINDHIEFIREKNGKYYSENYKFKVTTRQEQDIRLYYPRTYKKIKNNEFEFFYQEVNPKKERQSKLNI

42.5 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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