This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Belongs to the N(4)/N(6)-methyltransferase family.

580 amino acids

MELTIEEMLIKQKETGAHYTPTDLGDIIAKRLINELKKSGISGTKKIRGLDPSCGDGELLLSLNRIAKFNNIDNIELIGIDEDKEAIKEADFRLNEMGINDAKLTAGDFLDMVDLEGNLSLFDDDLSKIEPVDLIIANPPYVRTQVLGADRAQKLAKLFNLKGRVDLYHAFLVAMTLQLKPGGLIGVITSNKYLANSSGESIRQFLAENYDIIEIMDLGDTKLFSAAVLQAIFFGRKKVNKGIRQTAPANFYKIYEETDPSKTEVSIKFETLFGLLESSNTGVFNVDEKFYSVSCGKLIVPDSFKEPWVMATDEEYNWITNINNNSYCTIQDLCDVKVGIKTTADKVFIKSTWEELPDEIKPEVEVLKLLISTDHASKWRPLERIPNQKILYTHENVNGKKKAIDFTQYPHALAYLETHRETLEGRKYVIKAKRNWYEIWVPQNPDHWALPKIVFPDISPEPKFFYEDEGCCIDGNCYWIIPKEENNNDILFLILGISNTKYMTNYHDIAFNNKLYPGRRRYLTQYVSKYPLPNPEANYSQEIIDLVRELVFQNPNDERKIEIENQIENLTALAFGVERL

66.3 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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