This methylase recognizes the double-stranded sequence GTMKAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AccI endonuclease.

Belongs to the N(4)/N(6)-methyltransferase family.

540 amino acids

MIKTTEHIVNNSIERDYSKSISLEHRKKFAQFFTPFPIAYAMAKWILGNKQLKTVLEPAFGLGVFSRAILSQQKEINIKAFEVDETIFENAKEYFDDFENVNILLQDYMYNDWKNKYDGIICNPPYFKFHDYDNKNILKEIETNLKCKLNGFTNLYTLFLLKSIHQLSQNGRCAYIIPSEFLNSDYGKLVKTYLIKSKTLRHIIVIDFEENVFDDALTTASIILCANDNITDKVQFNNIQSLQDLSKIDEIINKYPNFLETEQTYNFSDLNPEIKWKAYYQKQNSIKFKNLVPFSTYAKVVRGIATGSNEYFTFNLSKAKEFNIDEQYLLPCICSAKDAKTSFFTKQDFEELKKSDKSVFLFNAQNSTDKNISSYIQKGESEEINKRFLTASRTPWYSLENRKPAPIWVSVFNRSGLRFIRNEANISNLTSYHCIIQNKQVVSEIDIDLLFAYLLTDTAKQIFEDNSRQYGNGLQKFEPNDLNKGMMLDLGLLDKQTSDEILNLYKEYKYLILDNKNGDEIINKIDKILTDKYSEKKHWA

63.1 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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