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Protein expression services for CmaA | L-allo-isoleucine:holo-[CmaA peptidyl-carrier protein] ligase

Description
Involved in the biosynthesis of the phytotoxin coronatine (COR) which mimics the plant hormone jasmonic acid isoleucine and promotes opening of stomata for bacterial entry, bacterial growth in the apoplast, systemic susceptibility, and disease symptoms. CmaA catalyzes the adenylation of L-allo-isoleucine (via the A domain) and the attachment of L-allo-isoleucine to the 4'-phosphopantetheine arm located within the T domain of CmaA. It can also use L-isoleucine, L-leucine and L-valine as substerates.
Family
Belongs to the ATP-dependent AMP-binding enzyme family.
Species
Pseudomonas savastanoi pv. glycinea
Length
595 amino acids
Sequence
MTSYHSHPPKAYRRFESVCTQAPNAIAVVHEGKPVTYQQLQTQVLERSEALIRQGLADHPYMPLMANRCLEYLITMLACCKLGITYVSIEPSTPSKRLIAVLEQLGCNHLLLLGQPTDLRPDPTLTCFRLDDCGTLCSDGPALRQPIRRRLDDASVITVMFTSGTTGVPKGVRISQDGLLNLVDNVQQQVQGKPRSYVHHSSIGFDAALFEVWVPLLTGACVTLQPSEFNIDALDHCVRAASCDVLLLTTSLFHLVAQHRLSMLEAVRVLYVGGEVLKPVHARALLLANPRITLVNGYGPTENTVFSTWYSLNKPEDAERDVIPIGQFLHQVHGKIVDAKLQEVEVGTPGELLLTGANLALGYLDEALTPTRFLQLPEGTYYRTGDYVIQDEHGMLFYQGRIDEQVKIKGFRVEIAEVEHALTQLPGVAQAVVQAHVMNDLENSLHAFIVFRHGSPTIEESKLMSLLGDRLPHYMVPRRIHYLAELPLTANGKVDKRSLQPPEKAAVVSPQAGSAVLEIWSGILGTRNLQLEHSIYGYGASSLSVVMAHSRINEILGRTTPFDEVARLSTFQEWVQYYATHADPVTSLRSQHGNH
Mass
65.9 kDa
Simulated SDS-PAGE
Western blot of CmaA recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make CmaA using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

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