Isopropyl malate synthase; part of the gene clusters that mediate the biosynthesis of AM-toxins, host-selective toxins (HSTs) causing Alternaria blotch on apple, a worldwide distributed disease (Probable). AM-toxins are cyclic depsipeptides containing the 3 residues 2-hydroxy-isovaleric acid (2-HIV), dehydroalanine, L-alanine which are common for all 3 AM-toxins I to III. The fourth precursor is L-alpha-amino-methoxyphenyl-valeric acid (L-Amv) for AM-toxin I, L-alpha-amino-phenyl-valeric acid (L-Apv) for AM-toxin II, and L-alpha-amino-hydroxyphenyl-valeric acid (L-Ahv) for AM-toxin III (Probable). AM-toxins have two target sites for affecting susceptible apple cells; they cause invagination of the plasma membrane and electrolyte loss and chloroplast disorganization (PubMed:22846083). The non-ribosomal peptide synthetase AMT1 contains 4 catalytic modules and is responsible for activation of each residue in AM-toxin (PubMed:10875335). The aldo-keto reductase AMT2 catalyzes the conversion of 2-keto-isovaleric acid (2-KIV) to 2-hydroxy-isovaleric acid (2-HIV), one of the precursor residues incorporated by AMT1 during AM-toxin biosynthesis, by reduction of its ketone to an alcohol (PubMed:15066029). The cytochrome P450 monooxygenase AMT3 and the thioesterase AMT4 are also important for AM-toxin production, but their exact function within the AM-toxin biosynthesis are not known yet (PubMed:17990954). Up to 21 proteins (including AMT1 to AMT4) are predicted to be involved in AM-toxin biosynthesis since their expression ishighly up-regulated in AM-toxin-producing cultures (PubMed:17990954).

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.

582 amino acids

MFEPYLTTPLLLKAANTPHHIPKMSRICSDRPSTLVKYGNRVQPFPFPERQWPNKVLKTAPVLFSTDLRDGNQSLPNPMTFEQKLSLYKLLVSIGFKEIEVAYPCANQAEFDFVRHLIETPGLIPEDVLIQVITPCQEETIKRAVESVRGAKQAILFTYLPSSDNYRDTVLKISEDDWVERARWGAAYARSITKDSEDPVVRSTRWTFNFGFEDFANARIGAIIRCTEAVRAEWNPSKDEKMIIGVASSVEASTPNVFADQIECLSGNISNRDTVRLTVHTHNDRGGAVASAELASLAGADRIEGCLFGNGERAGNMDLVVYALNLLTQGIEPGIDLSRLDEIRKVYEDITELPVHPRTPYSGAYYLKAFSGAHQDAISKGLRLRTSATQEGKPCAVWPAWRVPYLPLDPADIGRSLDDVVGINSQSGKGGVAWVVNLGLGLDLPPGLARTFSKAVKERSIHFGREMSSDEVCMAFLEEYQVLRAADKSDDDMIQAVLRTESPLVAALGQVVGLSSLSASITSHTLADDVLSYAAYANVTPEESKPSLWGVGLGVSLQQAKLRAVVSALQVSDGSMSWVRLA

63.9 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.

Make AMT7 using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

Order Here