Description
Involved in the biosynthesis of the azoxy antibiotic valanimycin, which has an antitumor activity (PubMed:9056232, PubMed:8990292, PubMed:9287340). Catalyzes the oxidation of isobutylamine to isobutylhydroxylamine via the formation of a flavin 4a-hydroperoxide (PubMed:9056232, PubMed:8990292, PubMed:9287340). Unlike other known N-hydroxylases, isobutylamine N-hydroxylase cannot carry out the reduction of the flavin cofactor and requires the NADPH-flavin oxidoreductase VlmR (PubMed:9056232). Also able to oxidize propan-1-amine, butan-1-amine, butan-2-amine and benzylamine (PubMed:9056232). It has a similar activity with either FMNH(2) or FADH(2) (PubMed:9056232).
Species
Streptomyces viridifaciens
Sequence
MRSLDAARDTCERLHPGLIKALEELPLLEREAEGSPVLDIFRAHGGAGLLVPSAYGGHGADALDAVRVTRALGACSPSLAAAATMHNFTAAMLFALTDRVIPPTDEQKKLLARVAPEGMLLASGWAEGRTQQDILNPSVKATPVDDGFILNGSKKPCSLSRSMDILTASVILPDETGQQSLAVPLIMADSPGISVHPFWESPVLAGSQSNEVRLKDVHVPEKLIIRGTPDDPGRLDDLQTATFVWFELLITSAYVGAASALTELVMERDRGSVTDRAALGIQLESAVGLTEGVARAVRDGVFGEEAVAAALTARFAVQKTLAAISDQAIELLGGIAFIKSPELAYLSSALHPLAFHPPGRTSSSPHLVEYFSGGPLEI
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service