Description
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.
Family
Belongs to the GatB/GatE family. GatE subfamily.
Species
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Sequence
MTELDYSKLGLKVGLEIHQQLNTNKKLFCDCSTTLDEKHHGTLQRYLRPSFSEIGEIDTAALFEWQKGKKYLYQIPFNSCLVEADEEPPHGLNREALAVVLAVAMSLESNIVDEIYVMRKIVIDGSDTTGFQRTSIVAMGGQVIVEGKKIGIQTIALEEDAARKISESANETMYSLDRLGIPLIEISTAPDITTPEEAEKVAFRIGQLLRLTGKVKRGIGTIRQDLNVSIQGGVKTEIKGVQRLELIPEIIKNEARRQYELLKIKDELVNKRGLSKTIVENEIKELELTHLFRNTNSKIIKKELEKGGLIYGIKFKGFKGIFGRELMPNRRFGTEIADYVRALAELGGIFHSDELPNYGITSEEVESVKKELGIGENDGFVLVIGDKEKLKVAITKIKERVLYAFVGVPKETRVALDDGTTKFMRPQPGSARMYPETDIIPIKIDESILNFAKSFVPENPETKLRKLIEMGLSKELATEILNSPRLDLFEELSKKYSPKVSPIVIATTLENYIKYAKSKGGDISVITDEVIEEIINALYNDKISKDSIQEILVDYSTSKKPIRNIVDNYAKITDEELNRIIDKILDENKDIINQKGEKAFNVIIGKVMNVVKGRAEGKKVVDTLKLKMKNYPRT
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service