Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.

Belongs to the GatB/GatE family. GatE subfamily.

608 amino acids

MDYRALGLKVGLEIHIQLNTRRKLFCHCPPVLRDDEPHFRLERRLHISLSELGAVDPAVVWEVRKRRRYVYEGYRDTTCLVELDEEPPHLPDEEALVTALGVAKMFNAKPFDELHVMRKIVVDGSNVSGFQRTVLVAYGGRAKILGYDIGVETIALEEDAARKMGEEGKAVIYRLDRLGIPLIEIATEPMSYSPQQVEEVAWIIGYSVKITGRAKRGVGTVRQDVNVSIAGGAKTEIKGVPDLSLIPKVIEYEAQRQVNLLKIAEELKKRGVASVELSTVDVTDVFSNTKSKLVKRVLEAGGRVVAVKAPGFRKLLGFEVQPGRRFGTELADYVRAWTELGGLLHSDELPGYGISADEVRAVEQRVGVDSYVLLMGVEAAELEEAARVVVDRLNAALRGVPEETRGANPDGTTRFLRPRPGAARMYPETDLPPIKVTFELLKRAEEVAKVSLEGKIAELTSMGLSKDLALRLVKSPHLEKFEEFVARFKSLPPQLIASIMLNTAKALAREGVEVSEEKLASVFEALENKVITKEAVEDVLRAMKPGESAAEVAKRLGLVRLPFEEVRKIVEEVAKEVGREKALGEVMRRYRGRVDAEDVRRALSELHF

67.6 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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