Description
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Family
Belongs to the amidase family. GatA subfamily.
Species
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Sequence
MTDTNVNIIRLTAAEIAAKVASGELTAVQVTEAHLARIDAVDEKVHAFLHVDREGALAQARAVDEKRERGEKLGPLAGVPLALKDIFTTEGIPTTVGSKILEGWIPPYDATLTKKLKAADVVILGKTNMDEFAMGSSTENSAYGPTGNPWDLSRIPGGSGGGSSAALASFEAPLAIGTDTGGSIRQPAAVTGTVGVKPTYGAVSRYGMVAFSSSLDQGGPCARTVLDAALLHEVIAGHDPLDSTSIDAPVPPVVEAARNGSVEGMRVGVVKQFRGEGYQAGVLQRFDESVALLKELGAEIVELDCPSFDLALSAYYLIAPSECSSNLARFDGLRYGVRVGDDGTRSAEEVTALTREAGFGDEVKRRIMLGTYALSSGYYDAYYGSAQKVRTLITRDFEKSFEQVDVIVSPTTPTTAFPIGERADDPMAMYLADLCTIPTNLAGNAAMSLPCGLAPEDNLPVGLQIIAPALKDDRLYKVGAAVEAAFVEKWGHPLIEEAPSL
Simulated SDS-PAGE

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service