Description
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Family
Belongs to the amidase family. GatA subfamily.
Species
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Sequence
MSLFDHKISELKRLIHNKEISISDLVDESYKRIHEVDGKVQAFLQLDEEKARAYAKELDEALDTRDEHGLLFGMPIGIKDNIVTKDLRTTCASKILENFDPIYDATVVERLHEAEAVTIGKLNMDEFAMGSSTENSGFKKTKNPWNLETVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRSVEDNAYLLQAISGVDKMDSTSANVDVPDYLSALTGDIKGLKIAVPKEYLGEGVSEEAKQSVLEALKVLESLGATWEEVSLPHSKYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRSEGFGNEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFANYDVIIGPTTPTPAFKIGEKTSDPLTMYANDILTIPVNLAGVPGISVPCGFANGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKPEL
Simulated SDS-PAGE

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service