Description
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Family
Belongs to the amidase family. GatA subfamily.
Species
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Sequence
MLNASLRQLSLLLSEKKISSTELTSEFLSRIKALNPDLNAFITIDEEKSLDQANVADKMIAAGRSTPLTGIPIAQKDIFCARGWLTTCGSKMLSNFVSPYDATVVERFDQAGMVNLGKTNMDEFAMGSSNETSYYGPVKNPWDRLAVPGGSSGGSACAVAARLAPAATGSDTGGSIRQPAALCGISGIKPTYGLVSRYGMIAFASSLDQGGPMAKSAEDLALLLNTMVGFDERDSTSLQRAEENYTQDLEKPVNGLRIGLPKEFFAEGMSSDVSNVIEAALAEYRKLGATFVEVSLPNSKLAVPVYYVLAPAEASSNLSRFDGVRYGYRTAQYSSLEDLYTKTRAEGFGEEVKRRILIGTYVLSHGYYDAYYLQAQKLRRLIAEDFRKAFEQCDLIMGPTTPTVAFNIGEKCDDPIQMYLSDIYTSTASLAGLPGMSIPAGFGSKNRPVGLHIIGNYFREAQMLNVAHRYQQVTNWHELTPPETSN
Simulated SDS-PAGE

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service