Description
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity).
Family
Belongs to the amidase family. GatA subfamily.
Species
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Sequence
MEILDMTVEQLRNAILDKHLKSEDIVKAYFDNIKRNEPEINAYITLCEDYALKEAKDVDKKIANGDKVGRLAGIPIAIKDNICTDGIKTTCASKMLYDFVPPYDATVIKKLKAEDAIIIGKVNMDEFAMGSSTENSAFKITKNPRDITRVPGGSSGGSAAVVAAKMAPISLGSDTGGSIRQPAAFCGVVGLKPTYGLVSRFGLIAFASSLDQIGPLGKTVKDCAELLEVISGEDELDNTSSKKHEKEDYLEGIDDGIKGMKIGMPKEFLNDGLDPEIRKCIDDTIEKLKSLGAEVCEMSLPITEEGLSAYYIISSAEASSNLARFDGIRYGYRPDDFEDVYDLMETSRSEAFGDEVKRRIMLGTYALSSGYYDAYYKRALKLKKKIKNEFKEAFENYDLILSPVSPVLPFKIGEKKADPLQMYLADIYTVNINLAGIPAISLPCSVSKEGLPIGLQLLGPHFGEKKIFRAARALEKER
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service