Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon.

Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

298 amino acids

MNPGYRGRFAPSPTGPLHFGSLVGALASWLDARAHGGTWLVRIEDLDGPRTVPGAADDILATLAHFGMTPDEPPIWQSTRDAAYTAALERLVAAGLVYPCGCTRKEIADSLRAAHERHTTLAYPGTCRTGLHGKPARAWRLRVPDGNDAVVTFDDRWQHAQSQNLATEVGDFVLKRADGQWAYQLAVVVDDADAGITHVVRGADLLDSTARQIYLQRCLGVPTPAYLHVPVVVDANGEKLSKQTGATALERDDPLPALRAAAAHLGLAADGDRPVHTIDAFYAAATDAWARRFGPRAG

32 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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