Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.

Belongs to the thymidylate synthase ThyX family.

270 amino acids

MEGPLTIPVLDKGFVRLVDQMGDDRAIVQAARVSYGEGTKTVREDAALIDYLMRHRHTSPFEMVVFKFHVKAPIFVARQWFRHRTASVNEISGRYSILKEEFYEPEAFRKQAKRNKQASEGALLDEEALALLRKVEQEAYGAYRALLEKGVAREMARMVLPLNLYTEFYWKQDLHNLFHFLKLRLAPEAQWEIRQYARAIAEIVKERVPLAWAAFEEHLLEGAFLSRTELRALRGLLTPEVYEKALSSLGLGGSRLKEALEKVFGPGEAL

31 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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