FAD-dependent monooxygenase; part of the gene cluster B that mediates the biosynthesis of austinol and dehydroaustinol, two fungal meroterpenoids (PubMed:22329759). The first step of the pathway is the synthesis of 3,5-dimethylorsellinic acid by the polyketide synthase ausA (PubMed:22329759). 3,5-dimethylorsellinic acid is then prenylated by the polyprenyl transferase ausN (PubMed:22329759). Further epoxidation by the FAD-dependent monooxygenase ausM and cyclization by the probable terpene cyclase ausL lead to the formation of protoaustinoid A (PubMed:22329759). Protoaustinoid A is then oxidized to spiro-lactone preaustinoid A3 by the combined action of the FAD-binding monooxygenases ausB and ausC, and the dioxygenase ausE (PubMed:22329759, PubMed:23865690). Acid-catalyzed keto-rearrangement and ring contraction of the tetraketide portion of preaustinoid A3 by ausJ lead to the formation of preaustinoid A4 (PubMed:22329759). The aldo-keto reductase ausK, with the help of ausH, is involved in the next step by transforming preaustinoid A4 into isoaustinone which is in turn hydroxylated by the P450 monooxygenase ausI to form austinolide (PubMed:22329759). Finally, the cytochrome P450 monooxygenase ausG modifies austinolide to austinol (PubMed:22329759). Austinol can be further modified to dehydroaustinol which forms a diffusible complex with diorcinol that initiates conidiation (PubMed:22234162, PubMed:22329759).

Belongs to the paxM FAD-dependent monooxygenase family.

479 amino acids

MSDTPTRKTDLRVIIVGGSVAGLTLAHCLANANIDHIVLEKRAEISPQEGAFLGIWPNGGRIFDQLGVYADLEKCTVPIHKMRVRFPDGVSFSSELPRQVQERFGYPIISLDRQKVLEILYNRYPAKSNIHVNKKVTEIRQTEREAQVLTADGAVYKGDLVVGADGIHSAVRAEMWRQAKDLVGRRDRQDVNHEIAAFTVEYACVFGISSPISGLESGEHVNSYSNGLCVITFHGKDGRVFWFILIKLQKRFIYPFTPRFSASDAAKICAEYANVPVWGDICVRDLWGNKTSVSMTALEEGLLETWRFKRVVLLGDSIHKMTPNIGQGANTAAEDAGVLASLLQRLSTSDSSATSCTIDAVLQEYASLRYERVKSTYQRAYFGARLHTRDDALKAFVGRYIFPRFRQQVLERTSQAIAGAPQVDFLPTPKRTGPGWSDYAGSPEVGAPTLPWLVISLPVLASMLCYLVYSSVFVTPIFP

53.2 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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