Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.

295 amino acids

MAAVVALGANVLSAKSIICDIEGTTTSISFVKDTLFPYALKHVEGYLKNNWNEEATKTVVTALREQAEEDKKAEVEGVVPIPTGDSEDIIPEIVKNVEWQMSLDRKTGSLKTLQGLVWAKGYKDGSIKGHVYDDVQKAFEQWTENGRKIYIYSSGSVDAQKLLFEHSEQGDLLKYLSGHYDTKIGAKREKESYTSILKNIESSPEEALFLTDVYAEAKAAKEAGLNVVLLDRPGNSELSEEERKDFPVIATFSDLSFAAETKEENGGATNGKRKIEETNDDVAEEDKAQVYPNKK

32.7 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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