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Protein expression services for RDT1 | Elsinochrome reductase 1

Description
Reductase; part of the gene cluster that mediates the biosynthesis of elsinochromes, pigments consisting of at least four interconvertible tautomers (A, B, C and D) that have a core phenolic quinone to which various side chains are attached and which play an important role in fungal pathogenesis (PubMed:18957608). The non-reducing polyketide synthase PKS1 was proposed to iteratively catalyze decarboxylation between acetyl-CoA and malonyl-CoA subunits for polyketide chain elongation. The released polyketide undergoes cyclization to form an aromatic ring, and proceeds via serial modification steps to produce the heptaketide back- bone of elsinochrome. As elsinochrome has a symmetrical structure, two identical heptaketides are fused to form a core 1,2-dihydrobenzo-perylene ring structure, which can then be successively modified to produce the various derivatives of elsinochrome. Some of these reactions may be cooperatively carried out, at least in part, by the products of RDT1, OXR1 and PKS1. PRF1, embedded within the elsinochrome cluster possibly functions to stabilize some of the biosynthetic enzymes required for elsinochrome production. As prefoldin is a hexamer containing 2 a and 4 b subunits, additional prefoldin subunits, whose coding genes may not immediately link to the elsinochrome biosynthetic gene cluster, are required to fulfill the chaperone function. In addition, no methyltransferase-coding gene exists within the biosynthetic gene cluster, even though elsinochrome has four methyl groups at positions C3, C7, C8 and C12. Apparently, the identified gene cluster does not contain the entire entourage of genes responsible for elsinochrome biosynthesis. Once elsinochrome is synthesized, it must be exported outside the fungal cells, which is probably accomplished by the ECT1 transporter, to avoid toxicity (PubMed:21199563).
Family
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Species
Elsinoe fawcettii
Length
267 amino acids
Sequence
MSALQALSTSRLDGKVALVTGSGRGIGAAIATELANRGASVVVNYANSPDAAQTVVDAIKKNGGDAIALQADVSDVKQTIKLFDDAVAHYGQLDIAVSNSGVVSFGHLKDVTEEEFDRVFAINTRGQFFVAREAYKHLSVGGRIILMGSITGQAKGVPKHTVYSGTKGAIETFVRCMAIDCGDKRITVNCVAPGGIKTDMYHAVCKEYIPNGENLTNEQVDEYAATWSPLNRVGQPIDVARVCGFLASQDGEWINGKVLGIDGAACM
Mass
28.2 kDa
Simulated SDS-PAGE
Western blot of RDT1 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make RDT1 using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

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