Description
Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (ManNAcXyl(2)GlcAMan(2)GalNAc in P.furiosus) from the lipid carrier dolichol-monophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation.
Family
Belongs to the STT3 family.
Species
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Sequence
MKIDKRLMVIVAIATLFRMIPFRLKYLVGSDPYFHLAYIEEALKAGEWFNFFTYAGGPWGLQVRLFHPLGLWATPAYIYKLFSFLGISLYTAFRVTPVIFGVLTVVFFYLSLKKLYNRDVAFIVGLFLGVNYGHIFRSMANYYRGDNYMLFWYSVALLGIALGLKTRSKYRYLFYLLPGIATGFASAFWQAYYPIFVFVLAGGLLLGVYAYLKSPKLFLDSILIVLSTGLGVLIANILGDKVGYGMLGYTDWMGKKVAETFGLEFGFIKDAYLLIHVKYLLPLSLVFLGFLIITKKLNPKIKVGVLVGGSILAFIVMLVKFPALKDLSTGFGTFREVPISETLPPTLDDLWRAYNIAIFLAALYILRLRKIRSGDAILLGYVITSLWMLRYWTRFLFTAAPAVAFLSGIGVYELTRRIKENKIRITSLGVVILLSSAFSLGEVYSVKPFMNENWEKALIFIRENSNENDIVLTWWDWGHFVTYYARRSPVAQGSPNSGVAGYYLGLVDNGWAQSLGVDYVIVSLYDILKFEAIVDTAKLSRKWENISRADYGVDFLKLTESTGSILRFDSQYSTLIVKEGNIRVILSGKVVYPREAIIESNGRIKNLKYPARSGVYVYVNLDYGYAILANEKAWETNLLRLFTQRTGENYELVYSDGGFVKVFRFVHPNVVFRGNKFILTGNGTGLGLYGYLDNGTLVFKKWYSVKNMQEFELPNNLNGSVVVRYVYTQGKIVLDRGIVRVKN
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service