Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with ctu1/atpbd3 that ligates sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at wobble position.

Belongs to the CTU2/NCS2 family.

523 amino acids

MSSEELPSCGINDNDINNTIPINTRKVQIIPNGNTQCVKCLYNVANNVKDKKLSKKEKKEQKLKEEENNNNNNEEPITQQQKPIGKPIINFRSEQLCWECYRELILKKFKLNIVKVRESKRDAEKLLVALSGGTCSSMLLELLKQCTEGSGKAKMFLDIKCVHIDESSITPYQNHNDTIEFLKEFNNVKLGFPNLEIIPLEDILGTVTPLGERTNQLKLQFAQLSSETSKEDLLLYYRNQLLIQVAHKLNCKKVILGTSSNRLAVQLVASTSKGRGFSVPNETSVIIEQPSNDIKFYQPMRDFLLKEIFIYYRHLNILPVPVMFSILNLKPKHSINTLCEDFLHCLQDISNQTVHTLLRSVDKLISPSIDSNYNCSICSSPLTSAEIKSLEKVILDNNNNINKENKNNNNNNNNNNNNNGCCSTTKTEDSSCCNKTEDNSSNNNNNNTGCCSSSSSTSTSSITVNKETLCYSCKILYRDFKSTPNIAPYIKENSKQLLTTSQLKNEIKDFLLNSDDDDDDEDN

59.1 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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