Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of sirodesmin PL, an epipolythiodioxopiperazine (ETP) characterized by a disulfide bridged cyclic dipeptide and that acts as a phytotoxin which is involved in the blackleg didease of canola (PubMed:15387811, PubMed:18272357, PubMed:19762440). SirD catalyzes the O-prenylation of L-tyrosine (L-Tyr) in the presence of dimethylallyl diphosphate (DMAPP) to yield 4-O-dimethylallyl-L-Tyr, and therefore represents probably the first pathway-specific enzyme in the biosynthesis of sirodesmin PL (PubMed:19762440, PubMed:21038099, PubMed:24083562). 4-O-dimethylallyl-L-Tyr, then undergoes condensation with L-Ser in a reaction catalyzed by the non-ribosomal peptide synthase sirP to form the diketopiperazine (DKP) backbone (PubMed:18272357). Further bishydroxylation of the DKP performed by the cytochrome P450 monooxygenase sirC leads to the production of the intermediate phomamide (PubMed:27390873). This step is essential to form the reactive thiol group required for toxicity of sirodesmin PL (PubMed:27390873). The next steps of sirodesmin biosynthesis are not well understood yet, but some predictions could be made from intermediate compounds identification (PubMed:18272357). Phomamide is converted into phomalizarine via oxidation, probably by sirT (PubMed:18272357). Further oxidation, methylation (by sirM or sirN) and reduction steps convert phomalizarine to deacetyl sirodesmin (PubMed:18272357). Finally, acetyltransferase sirH probably acetylates deacetyl sirodesmin to produce sirodesmin PL (PubMed:18272357).

Belongs to the cytochrome P450 family.

522 amino acids

MSTLQDFQIAPAILHRVTVRDASAILFCTLLTVFLFISQGTVRGVKRVGKSRLRTLVTGETPLRFDLEKYGYLGYQQFSKKANKPFLVKIYGLDHYVLPVKYLDSLKTVDHHRLSFAQSLNDFLNVDASLGDLVTHSDMEIAVVTKHLNPRLTTLTPVLVDEANFAFEKELGKLETWKTVNALFLSAFLTNRTSGRVLVGDLCRDDNYLHAMMKYTESVFSSGVAFNGIPLGPFRKIVYYLGARQHRRDLDNAAALVLPEIKRRMAAQAEDPNCRKENDAIQWNLDLPLASPKEGLPLRHAHRVLHLSFAATGTVAILITHMIYNVLMYPEYLEPLREEVMACTKAHGGWTEKAMNEMWKLDSFIRETLRVQPPSVFTAYRTVKNQPFTFPDGFTLPVGSRITFPTLPVGLDPENYESASEFDGFRFFRKREEARLAKKAYNWGATKIDSSFLPFGYGNQACPGRFFGVRKTKILFGKLINDYDFSWAEPRSARPENIVIEGQILVNPTPEIKIKSRVAAGM

59.1 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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